Written by Alisa - 4 Minutes reading time

Exposing how uberculosis evades the immune system

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Tuberculosis, caused by the bacterium Mycobacterium tuberculosis (Mtb), kills upwards of 1.6 million people a year, making it one of the leading causes of death by an infectious agent worldwide. However, it is not fully understood how Mtb evades the immune system. Now, a team of researchers from the University of Massachusetts (UMass), Amherst, and Seattle Children’s Research Institute report that prior exposure to a genus of bacteria called Mycobacterium seems to remodel the first-line defenders in the body’s immune system. They also found that how those cells are remodeled depends on exactly how the body is exposed.

Their findings are published in PLOS Pathogens in an article titled, “Exposure to Mycobacterium remodels alveolar macrophages and the early innate response to Mycobacterium tuberculosis infection.”

The study suggests that a more integrated treatment approach that targets all aspects of the immune response could be a more effective strategy against tuberculosis.

“We breathe in thousands of liters of air every day,” said Alissa Rothchild, assistant professor in the veterinary and animal sciences department at UMass Amherst and the paper’s senior author. “This essential process makes us incredibly vulnerable to inhalation of all sorts of potentially infectious pathogens that our immune systems have to respond to.”

Macrophages are the first-line defenders in the tissues that recognise and destroy pathogens and also call for backup. In the case of the lungs, these macrophages are called alveolar macrophages (AMs). They live in the lung’s alveoli, the tiny air sacs where oxygen passes into the bloodstream—but, as Rothchild has shown in a previous paper, AMs don’t mount a robust immune response when they’re initially infected by Mtb.

“Mtb takes advantage of the immune response,” added Rothchild, “and when they infect an AM, they can replicate inside of it for a week or longer. They effectively turn the AM into a Trojan Horse in which the bacteria can hide from the body’s defenses.”

“But what if we could change this first step in the chain of infection?” Rothchild continued. “What if the AMs responded more effectively to Mtb? How could we change the body’s innate immune response? Studies over the last 10 years or so have demonstrated that the innate immune system is capable of undergoing long-term changes, but we are only beginning to understand the underlying mechanisms behind them.”

Dat Mai, a research associate at Seattle Children’s Research Institute and the first author of the paper, Rothchild, and their colleagues designed an experiment using two different mouse models. The first model used the BCG vaccination, one of the world’s most widely distributed vaccines and the only vaccine used for tuberculosis. In the second model, the researchers induced a contained Mtb infection, which they previously showed protects against subsequent infections in a form of concomitant immunity.

The researchers then challenged the mice with aerosolised Mtb and infected macrophages were taken from each mouse model for RNA sequencing weeks after exposure.

While both sets of AMs showed a stronger pro-inflammatory response to Mtb than AMs from unexposed mice, the BCG-vaccinated AMs strongly turned on one type of inflammatory program, driven by interferons, while the AMs from the contained Mtb infection turned on a qualitatively different inflammatory program. Other experiments showed that the different exposure scenarios changed the AMs themselves, and that some of these changes seem to be dependent on the greater lung environment.

“What this tells us,” said Rothchild, “is that there’s a great deal of plasticity in the macrophage response, and that there’s potential to therapeutically harness this plasticity so that we can remodel the innate immune system to fight tuberculosis.”

Kevin Urdahl, PhD, professor of pediatrics at Seattle Children’s Research Institute, lead PI for this IMPAc-TB consortium, and one of the paper’s co-authors, added that, “The overall goal of the program is to elucidate how the immune system effectively controls or eradicates the bacteria that causes tuberculosis so that effective vaccines can be developed. This is an important part of the larger IMPAc-TB program because we will be assessing the responses of human alveolar macrophages recovered from individuals who have recently been exposed to Mycobacterium tuberculosis in a TB-endemic region. The findings of Rothchild’s team will help us interpret and understand the results we obtain from the human cells.”

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